Figure EV5. ER‐HRP and cytoplasmic HRP .

• A, B
  Cells that stably express HRP in the ER (A) or at cytoplasm (B). Scale bar: 10 μm.
• C–F
  DAB polymerization after treatment with DAB/H₂O₂ for 24 h at 37°C (C), percentage of cells with DAB aggregates (D), and quantification of dead cells (PI⁺ cells) (E, F). Scale bar: 20 μm.

Data information: Bars indicate means ± SD of triplicates of one representative experiment out of two repeats. Asterisks indicate significant differences with respect to untreated cells, whereas hashes indicate significant differences between WT cells and HRP‐expressing cells, ***P < 0.001, ^## P < 0.01, ^### P < 0.001 (two‐way ANOVA).