Figure 4. Supernumerary DAL neurons are functional.
-
A, BPictures of adult WT and expanded DAL lineages showing expression of GABA in a subset of Poxn+ cells (maximum intensity projections of a few optical sections; split green, magenta, and blue channels). (A) Lineages expanded with the en‐GAL4 driver alone, in which GFP does not label the EB, although thus structure is discernible by GABA staining (arrow). (B) Lineages expanded and permanently labeled with en>act>GAL4, in which the EB is clearly visible (arrows). Note that there are GABA‐expressing cells outside the DAL domain (which is outlined by dotted lines in the magenta channel for clarity).
-
CSchematic representation of the EB and bulb (bu), which are neuropil structures deriving from projecting GABAergic DAL neurons (magenta spheres). Note that the DAL cell bodies and the EB are located in different antero‐posterior planes and that there are GABA‐expressing cell bodies outside the DAL domain. Also, most of the cell body expansion occurs in an antero‐posterior direction (see Movies EV3 and EV4) so volume difference is not fully captured in maximum intensity projections.
-
DRelative intensity of GCaMP6f expression in adult DAL lineages for control (black, solid line is mean, and shaded area is SEM) and experimental (green) genotypes over 7 min of recording, including response to picrotoxin (added at 1 min and washed out at 6 min: gray background), each normalized to their own maximum intensity. Histograms represent the mean integration of the area under the curves (n = 5 CNSs; details in source data) during picrotoxin application and error bars SEM (no statistical difference; unpaired two‐tailed t‐test: P = 0.6068).
-
EMaximum intensity projections of GCaMP6f expression in adult DAL lineages of WT and expanded DAL lineages with pros knock‐down, with color outline of five example cells per genotype whose individual normalized calcium traces are shown (details in source data).