Figure EV5. Additional calcium responses of DAL neurons and supernumerary DAL neurons to picrotoxin (related to Fig 4).

Maximum intensity projections of GCaMP6f expression in adult DAL lineages of WT and expanded DAL lineages with pros knock‐down over 7 min of recording, including response to picrotoxin (added at 1 min and washed out at 6 min: gray background), with color outline of five exemplary cells per genotype whose individual normalized calcium traces are shown. There is biological variability in both control and pros knock‐down brains, which we tried to convey in the figures by depicting a range of responses. Again, we could not detect any individual neuron that did not respond to the picrotoxin treatment suggesting that supernumerary DAL neurons are functional.