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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Anal Chem. 2018 Jan 12;90(3):1941–1950. doi: 10.1021/acs.analchem.7b04032

Figure 1.

Figure 1

Generation and characterization of arrayed colonoids and comparison to embedded colonoids. A.) Work flow for generating the arrayed colonoids. B.) Brightfield image of an individual colonoid within the array. C.) F-actin (green) and Hoechst (blue) stained cryo-sectioned image of an arrayed colonoid. D.) Cryo-sectioned brightfield image of an arrayed colonoid. E & F.) EdU (green), MUC2 (red) and Hoechst (blue) stained images of an arrayed colonoid (E) and embedded colonoid (F) G.) Percent normalized area positive for EdU or MUC2 fluorescence in arrayed and embedded organoids. All scale bars represent 75 μm.