Table 3.
IL-23R expression on immune cells in mice
| Immune cells | Organ | Mouse | Methods | References |
|---|---|---|---|---|
| γδ T (γδ+) | Spleen, LP | Naïve IL-23R GFP B6 | qPCR, FC | Chognard et al. 2014 |
| γδ T (γδ+) | Lung | Naïve IL-23R GFP B6 | FC | Paget et al. 2015 |
| γδ T (γδ+) in vitro cultured | Spleen | Naïve B6 | qPCR, FC | Sutton et al. 2009 |
| γδ T (γδ+) | LN, LP | Naïve IL-23R GFP B6 | FC | Awasthi et al. 2009 |
| γδ T (CD3+ TCRδ+) | Spleen, LN | EAE B6 mice | qPCR, FC | Raverdeau et al. 2016 |
| Memory T cells (DX5− TCRβ+ CD62L−) | Spleen | Naïve B6 | qPCR | Rachitskaya et al. 2008 |
| CD4+ T cells (CD3+ CD4+) | LP | Naïve IL-23R GFP B6 | FC | Chan et al. 2014 |
| CD4+ T (CD4+ TCRβ+) | Spleen, LN | Naïve IL-23R GFP B6 | FC | Awasthi et al. 2009; Chognard et al. 2014 |
| Th17 cells (CD4+) cultured in in vitro Th17 conditions | Spleen | Naïve B6 | qPCR | Ciric et al. 2009; El-Behi et al. 2011 |
| LTi cells (CD3− CD4+) | LP | Naïve IL-23R GFP B6 | FC | Awasthi et al. 2009 |
| Treg (CD4+ FOXP3+) | Tumor, DLN | B16, MC38 and MB49 tumor-bearing B6 | FC | Kortylewski et al. 2009 |
| Tc17 (CD8+) Cultured in in vitro Tc17 conditions | Spleen | Naïve B6 | qPCR | Ciric et al. 2009 |
| B cells (CD19+ B220+) | Spleen, LP | Naïve IL-23R GFP B6 | FC | Chognard et al. 2014 |
| NKT (DX5+ TCRβ+) | Spleen | Naïve B6 | qPCR | Rachitskaya et al. 2008 |
| NKT (α-GC/CD1d tetramer+ TCRβ+ NK1.1−) stimulated with anti-CD3 and anti-CD28 | Thymus | Naïve B6 | qPCR | Coquet et al. 2008 |
| DC (CD11c+) | Spleen, LN | Naïve B6, naïve IL-23R GFP B6 | qPCR, FC | Awasthi et al. 2009, El-Behi et al. 2011 |
| Macrophage (CD11b+) | LN, LP | Naïve IL-23R GFP B6 | FC | Awasthi et al. 2009 |
| Inflammatory macrophage (CD4− CD11b+ CD45+) | CNS | EAE B6 | qPCR | Cua et al. 2003 |
| Neutrophil thioglycollate induced | Peritoneal cavity | Naïve B6 | qPCR | Chen et al. 2016 |
| Neutrophil (Ly6G+) | BM | Naïve B6 | qPCR, FC | Taylor et al. 2014 |
| Neutrophils (stimulated) | Colon | DSS-treated B6 | qPCR | Zindl et al. 2013 |
| LTβR+ cells | LP | DSS-treated mice | qPCR | Macho-Fernandez et al. 2015 |
| ILC3 (NKp46+ CD127+ CD117+ CD49b−) | Spleen | Naïve IL-23R GFP B6 | FC | Chognard et al. 2014 |
| ILC3 (Thy1+ NKp46+ CD3−) | LP | Naïve IL-23R GFP mice | FC | Chan et al. 2014 |
| LTi-like cells (CD3− CD127+ CD117+) | Spleen | Naïve IL-23R GFP mice | FC | Chognard et al. 2014 |
| LTi (Thy1+ cKIT+ NKp46− CD3−) | LP | Naïve IL-23R GFP mice | FC | Chan et al. 2014 |
| Thymocytes (Thy1.2+) | Thymus | Naïve B6 | qPCR, FC | Li et al. 2014 |
| Thymic epithelial cells | Thymus | Naïve B6 | qPCR | Li et al. 2014 |
FC, Flow cytometry; DLN, draining lymph node; LN, lymph node; LP, lamina propria; EAE, experimental autoimmune encephalomyelitis; B6, C57BL/6 mice; Treg, T regulatory cell; LTi, lymphoid tissue inducer cell; CNS, central nervous system; DSS, dextran sodium sulfate; LTβR, lymphotoxin β receptor; ILC3, type 3 innate lymphoid cells; BM, bone marrow; NKT, natural killer T cell; DC, dendritic cell; GFP, green fluorescent protein; IL, interleukin; qPCR, real-time polymerase chain reaction.