Fig 5. CAIX ablation decreases UFH-001 cell and tumor growth.

CAIX was ablated using CRISPR/Cas9. Two probe sets are shown compared to the empty vector control. Panel A. Western blots were probed for CAIX and GAPDH. Panel B. Cell growth of knockdown cells and empty vector controls was evaluated using the MTT assay. Panel C. Orthotopically implanted cells (empty vector controls and knockdown cells) were monitored for tumor growth in NOD/SCID mice. Asterisks denote statistical significance (** p < 0.01).