Fig 3. Cyp7b1, a rate-limiting enzyme of bile acid synthesis, is increased in whole-skin of SKO mice.

(A) Total bile acids are increased by 50% in SKO mice relative to controls. (B) The rate-limiting enzymes for hepatic bile acid synthesis cyp7a1 and cyp7b1 are unchanged whereas cyp7b1 in skin is increased. (C) Protein levels of cyp7a1 and cyp7b1 in liver and skin reflect mRNA levels. (D) Cyp7b1 protein expression is increased and localized around hair follicles.