Fig 4. Assessment of glucose tolerance in C57BL/6 mice at the end of 4 weeks of normal protein diet (NPD, control diet) or low protein diet (LPD, malnutrition diet) feeding during the first burden/malnutrition period, 4 weeks of chow diet feeding during the recovery period, and 16 weeks of chow or HFHC diet feeding during the second burden period.

(A, C, E) All intraperitoneal glucose tolerance tests (IPGTT) were performed during 2 h after glucose injection in mice that were fasted for 6 h. (B, D, F) AUC for the IPGTTs was calculated from 0 to 120 min. The LPD-fed mice following recovery and also LPD/HFHC mice demonstrated increased AUC (glucose) relative to the matched control mice. The NPD/HFHC-fed mice also had higher increased AUC relative to matched control mice. There was no statistical difference in the AUC between NPD-fed and LPD-fed mice at the end of 4 weeks of malnutrition period (All data are presented as means ± SD, n ≥ 4 mice per group, *P < 0.05 vs. NPD-fed mice following recovery, ^#P < 0.05 vs. NPD/HFHC-fed mice, ^§P < 0.05 vs. LPD/HFHC-fed mice, Tukey’s post hoc test, One-way ANOVA).