| Housekeeping gene |
| 18S |
5’-AGTCCCTGCCCTTTGTACACA-3’ |
5’-CGATCCGAGGGCCTCACTA-3’ |
| Fibrogenic genes |
| Collagen α1(I) |
5’-TAGGCCATTGTGTATGCAGC-3’ |
5’-ACATGTTCAGCTTTGTGGACC-3’ |
| Collagen α1(IV) |
5’-CACATTTTCCACAGCCAGAG-3’ |
5’-GTCTGGCTTCTGCTGCTCTT-3’ |
| PAI-1 |
5’-GCCAGGGTTGCACTAAACAT-3’ |
5’-GCCTCCTCATCCTGCCTAA-3’ |
| TGFβ1 |
5’-GTGGAAATCAACGGGATCAG-3’ |
5’-ACTTCCAACCCAGGTCCTTC-3’ |
| TIMP1 |
5’-AGGTGGTCTCGTTGATTTCT-3’ |
5’-GTAAGGCCTGTAGCTGTGCC-3’ |
| α-SMA |
5’-AAACAGGAATACGACGAAG-3’ |
5’-CAGGAATGATTTGGAAAGGA-3’ |
| Proinflammatory cytokines |
| TNFα |
5’-AGGGTCTGGGCCATAGAACT-3’ |
5’-CCACCACGCTCTTCTGTCTAC-3’ |
| IL-1α |
5’-CCAGAAGAAAATGAGGTCGG-3’ |
5’-AGCGCTCAAGGAGAAGACC-3’ |
| IL-1β |
5’-GGTCAAAGGTTTGGAAGCAG-3’ |
5’-TGTGAAATGCCACCTTTTGA-3’ |
| MCP-1 |
5’-ATTGGGATCATCTTGCTGGT-3’ |
5’-CCTGCTGTTCACAGTTGCC-3’ |
| Inflammasome component |
| NLRP3 |
5’-AAGTAAGGCCGGAATTCACC-3’ |
5’-AAAATGCCTTGGGAGACTCA-3’ |
| Marker of inflammation (infiltration of inflammatory cells) |
| CD68 |
5’-ACCGCCATGTAGTCCAGGTA-3’ |
5’-ATCCCCACCTGTCTCTCTCA-3’ |
| F4/80 |
5’-CATAAGCTGGGCAAGTGGTA-3’ |
5’-GGATGTACAGATGGGGGATG-3’ |
| Markers of oxidative stress |
| CYP2E1 |
5’-AGGCTGTCAAGGAGGTGCTAC-3’ |
5’-GTTTCCCCATTCCCCAGTC-3’ |
| Nrf2 |
5’-TCTTCCATTTACGGAGACCCA-3’ |
5’-GATTCACGCATAGGAGCACTG-3’ |
| Nqo1 |
5’-TCAACTGGTTTACAGCATTGGC-3’ |
5’-GCTTGGAGCAAAATAGAGTGGG-3’ |
| Gstp1 |
5’-ACCCTGCTGTCCCAGAACC-3’ |
5’-GCGAGCCACATAGGCAGAG-3’ |
| Ketohexokinase (KHK) primers |
| KHK-A |
5’-TTGCCGATTTTGTCCTGGAT-3’ |
5’-CCTCGGTCTGAAGGACCACAT-3’ |
| KHK-C |
5’-TGGCAGAGCCAGGGAGAT-3’ |
5’-ATCTGGCAGGTTCGTGTCGTA-3’ |
