Fig 3. Heat pulse entrainment phenotypes of NCU05950 deletion mutant.

(A) Control (pale blue), uv90 mutant (pale green) and NCU05950^KO (dark green) strains were grown at 22°C and entrained to repeated 2-hour pulses of 32°C at intervals of T hours. The normalized conidiation density of an average “day” (one T-cycle) is plotted against time after the beginning of the heat pulse. T-cycles were 16 h (top row), 20 h (middle row) or 24 h (bottom row). Thin lines are ± 1 S.E.M. Six replicate tubes were averaged for each trace, either 2 or 4 cycles from each tube, so N = 12 or 24 cycles averaged. All strains, including control, are also csp-1; ras^bd chol-1; frq^null. Growth media contained 100 μM choline to repair the chol-1 defect, which is not relevant to this experimental design. (B) Comparison of different T-cycles for the heat pulse entrainment of NCU05950 deletion mutant. Data are re-plotted from (A). T-cycles were 16 h (pink), 20 h (blue) or 24 h (orange). Arrows indicate peak times.