Fig 4. Rescue of the heat pulse entrainment phenotype of the uv90 mutant by the NCU05950 gene.

Control (pale blue), uv90 mutant (pale green) and uv90 mutant carrying a wild type copy of the NCU05950 gene (dark blue) were grown at 22°C and entrained to repeated 2-hour pulses of 32°C at intervals of T hours. The normalized conidiation density of an average “day” (one T-cycle) is plotted against time after the beginning of the heat pulse. T-cycles were 20 h (top row) or 24 h (bottom row). Thin lines are ± 1 S.E.M. Five replicate tubes were averaged for each trace, 2 cycles from each tube, so N = 10 cycles averaged. All strains, including control, are also csp-1; ras^bd chol-1; frq^null. Growth media contained 100 μM choline to repair the chol-1 defect, which is not relevant to this experimental design.