Fig 6. Rescue of the phenotype of the NCU05950 deletion mutant by the NCU05950-GFP and–FLAG fusion proteins.

All strains, including control, have the csp-1; ras^bd chol-1 genetic background. All strains except the control also carry the NCU05950 deletion allele (NCU05950^KO). GFP- and FLAG-tagged fusion genes were inserted at the his-3 locus of a NCU05950^KO strain. The pCCG fusion gene was expressed from the high-expression ccg-1 promoter. Other fusion genes were expressed from the native NCU05950 promoter. Δ1, Δ7 and Δ10: The FLAG fusion protein of NCU05950 expressed from the native NCU05950 promoter was modified by deleting either 1, 7 or 10 amino acids after the initial methionine at the N-terminus. Strains were grown with (+, left panel) or without (-, right panel) 100 μM choline in the medium. Two representative replicate tubes for each condition are shown. Growth is from left to right. White bars indicate average growth in 24 hours. Periods and growth rates are reported in S7 Table.