Table 2. Mismatch Data for ONs Containing Modified Monomer in the Center (ON3U–ON3Z).
| mismatch
data against MeC (5′-side of the linkage) |
mismatch
data against T (3′-side of the linkage) |
||||||
|---|---|---|---|---|---|---|---|
| Tma | ΔTmb |
Tma | ΔTmb |
||||
| match | mismatch |
match | mismatch |
||||
| ON code | ON sequence (5′–3′) | MeC–G | MeC–U | MeC–A | T–A | T–C | T–G |
| ON3U | 5′-CTC A MeCpT A TCT GCT | 55.1 | –17.7 | –16.1 | 55.1 | –12.3 | –3.4 |
| ON3V | 5′-CTC A MeCLpTL A TCT GCT | 66.0 | –18.1 | –17.2 | 66.0 | –12.9 | –4.6 |
| ON3W | 5′-CTC A MeCtT A TCT GCT | 49.6 | –17.0 | –15.2 | 49.6 | –10.7 | –2.1 |
| ON3X | 5′-CTC A MeCtTL A TCT GCT | 57.1 | –16.9 | –15.5 | 57.1 | –13.8 | –5.6 |
| ON3Y | 5′-CTC A MeCLtT A TCT GCT | 49.8 | –17.1 | –16.3 | 49.8 | –11.3 | –4.2 |
| ON3Z | 5′-CTC A MeCLtTL A TCT GCT | 58.1 | –17.3 | –16.8 | 58.1 | –15.2 | –6.8 |
a
Melting temperatures (Tm) were obtained from the maxima of the first derivatives of the melting curves (A260 vs temperature) recorded in a buffer containing 10 mM phosphate and 200 mM NaCl at pH 7.0 using 3.0 μM concentrations of each strand.
b
ΔTm = change in Tm for a mismatch duplex relative to the corresponding matched duplex. p = phosphodiester backbone. L = LNA nucleotide. t = triazole backbone. Matched RNA target: 5′-AGCAGAUAGUGAG. Mismatch RNA targets: 5′-AGCAGAUAUUGAG (MeC–U mismatch), 5′-AGCAGAUAAUGAG (MeC–A mismatch), 5′-AGCAGAUCGUGAG (T–C mismatch), 5′-AGCAGAUGGUGAG (T–G mismatch).