Table 3. Thermal Melting (Tm) Data for the DNA/DNA Duplexes.
|
Tma (ΔTmb) |
||||||
|---|---|---|---|---|---|---|
| ON code | ON sequence (5′–3′) | B = MeCpT (U) | MeCtT (W) | MeCtTL (X) | MeCLtT (Y) | MeCLtTL (Z) |
| ON1 | 5′-CTC ACT ATC TGB | 54.5 | 53.7 (−0.8) | 52.8 (−1.7) | 53.1 (−1.4) | 53.5 (−1.0) |
| ON2 | 5′-BCA CTA TCT GCT | 53.8 | 50.9 (−2.9) | 49.1 (−4.7) | 49.4 (−4.4) | 49.6 (−4.2) |
| ON3 | 5′-CTC ABA TCT GCT | 54.9 | 47.5 (−7.4) | 51.4 (−3.5) | 44.4 (−10.5) | 53.4 (−1.5) |
| ON4 | 5′-CTC ACT ATB GCT | 54.4 | 49.7 (−4.7) | 51.0 (−3.4) | 45.0 (−9.4) | 52.1 (−2.3) |
| ON5 | 5′-CTC ABA TBG CT | 55.9 | 41.0 (−14.9) | 48.6 (−7.3) | 34.2 (−21.7) | 51.4 (−4.5) |
| ON6 | 5′-BCA BAT BGB | 58.0 | 37.6 (−20.4) | 45.8 (−12.2) | <−30.0 | 48.3 (−9.7) |
a
Melting temperatures (Tm) were obtained from the maxima of the first derivatives of the melting curves (A260 vs temperature) recorded in a buffer containing 10 mM phosphate and 200 mM NaCl at pH 7.0 using 3.0 μM concentrations of each strand.
b
ΔTm = change in Tm for a modified duplex relative to the unmodified duplex 5′-CTC ACT ATC TGMeCT. p = phosphodiester backbone. L = LNA nucleotide. t = triazole backbone.