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. Author manuscript; available in PMC: 2019 Apr 9.
Published in final edited form as: Cancer Cell. 2018 Apr 2;33(4):676–689.e3. doi: 10.1016/j.ccell.2018.03.007

Figure 6. Chromosome 3p lung cells initially have slower proliferation, but normalize over time.

Figure 6

(A) AALE cells were transfected, and one day later cells were selected with puromycin for cells that had incorporated transfected DNA. Cells were single cell cloned to isolate chr_3p deleted clones, and assayed before and after extensive passaging.

(B) Proliferation curves were generated using CellTiter-Glo over 6 days (x-axis). Y-axis is relative luminescence units, normalized to Day 0. Data plotted are means with error bars +/- standard deviation. (* = p value < 0.01, ** = p value < 0.001) Cells were from Timepoint A.

(C) Heatmap of normalized enrichment scores for Hallmark gene sets in GSEA.

(D) Proliferation curves were generated using CellTiter-Glo over 6 days (x-axis). Y-axis is relative luminescence units, normalized to Day 0. Data plotted are means with error bars +/- standard deviation. Cells were from the higher passage population.

(E) Karyotype from one of the chromosome 3p deleted clones. Chromosome 3 is circled, and arrows point to chromosomal abnormalities.

(F) During passaging, cells have gained additional changes (*) to adapt to chr_3p deletion.

See also Figure S4.