10 μL	gel purified PCR product (or 5 μL of PCR cleanup reaction, 50–150 ng DNA)
2 μL	dCTP or dGTP (25 mM stock) – see Table 1
2 μL	T4 DNA Pol 10× Buffer
1 μL	100 mM DTT
0.4 μL	EMD/Novagen T4 DNA pol
4.6 μL	(or 9.6μL) H2O