| 10 μL | gel purified vector |
| 2 μL | dGTP (25 mM stock) |
| 2 μL | T4 DNA pol 10× Buffer |
| 1 μL | 100 mM DTT |
| 0.4 μL | EMD/Novagen T4 DNA pol |
| 4.6 μL | H2O |
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