Table 4. Summary of genotype frequencies in M. m. musculus backcross.
Crosses | AA | AB | Total | %AB | P-value | |
---|---|---|---|---|---|---|
Region 1 | musculusCZII × PWK sire | 100 | 93 | 195 | 47.7 | 0.614 |
140,089,156– | females | 54 | 50 | 106 | 47.2 | 0.695 |
141,037,913 bp | males | 46 | 43 | 89 | 48.3 | 0.75 |
musculusPWK × CZII sire | 144 | 157 | 301 | 52.2 | 0.454 | |
females | 77 | 83 | 160 | 51.9 | 0.635 | |
males | 67 | 74 | 141 | 52.5 | 0.556 | |
Total | 244 | 250 | 496 | 50.4 | 0.787 | |
Region 2 | musculusCZII × PWK sire | 97 | 97 | 195 | 49.7 | 1 |
148,602,050– | females | 53 | 53 | 106 | 50 | 1 |
151,609,913 bp | males | 44 | 44 | 89 | 49.4 | 1 |
musculusPWK × CZII sire | 138 | 162 | 301 | 53.8 | 0.166 | |
females | 73 | 86 | 160 | 53.8 | 0.303 | |
males | 65 | 76 | 141 | 53.9 | 0.354 | |
total | 235 | 259 | 496 | 52.2 | 0.28 |
To test for TRD within M. m. musculus, female musculusPWK were crossed to reciprocal interstrain F1s between musculusCZII and musculusPWK. Offspring were genotyped for two regions inside the introgressed TRD on Chr 4. AA and AB represent offspring genotype.