Figure 4.
Loss of Su(Hw) causes defects in spermiogenesis. (A) Representative confocal images of 1-, 3-, and 7-day-old su(Hw)+/+ and su(Hw)2/v testes stained with antibodies against polyglycylated tubulin (PolyG Tub; red, marks sperm tails), cleaved caspase 3 (Cas3, yellow, marks ICs), and phalloidin (blue, marks actin in ICs and elsewhere in the testis). Bar, 20 μm. Asterisk marks anterior of testis. (B and C) Quantification of su(Hw)2/v phenotypes as males age. End width: width of the posterior end of the testes leading into the seminal vesicle (S.V.). Numbers above data sets correspond to the number of testes examined. ** P < 0.01, **** P < 0.0001, ns, not significant, Student’s t-test.