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. 2018 May 8;209(3):757–772. doi: 10.1534/genetics.118.301088

Figure 9.

Figure 9

Cyst-cell-restricted Su(Hw) rescues testis morphology and male fertility. (A) Confocal images of 1-day-old testes isolated from su(Hw)+/+, su(Hw)2/v and C587-gal4 >UAS-su(Hw), su(Hw)2/v males stained with antibodies against Vasa (red) and Su(Hw) (green). Blue arrowheads point to cyst cells; white arrowheads point to germ cells. Bar, 20 μm. (B) Confocal images of 3-day-old testes isolated from su(Hw) +/+, su(Hw)2/v and C587-gal4 > UAS-su(Hw), su(Hw)2/v males stained with antibodies against spermiogenesis markers, PolyG tubulin (polyGTub; red), phalloidin (blue), and DAPI (white). Asterisks indicate the anterior tip of the testis. Bar, 50 μm. (C) Fertility outcomes of males of the indicated genotype tested in the sperm exhaustion assay. Axes are as described in Figure 1. Bars indicate the variation of fertility from three biological replicates (* P < 0.05, **** P < 0.0001, NS, not significant, Student’s t-test). The total number (n) of males tested is shown below the graph. S.V., seminal vesicle.