Table 3. Transposon insertions rare in YLR352W/LUG1 and NEDDylation-related genes.

Gene					Total reads			Unique inserts
					[URE3] − adenine	[ure-o] + adenine	[URE3] + adenine	[URE3] − adenine	[ure-o] + adenine	[URE3] + adenine
Genome total in ORFs					57,042,201	45,431,202	55,692,869	303,010	710,420	627,085
F-box protein
LUG1	YLR352W				1	9,882	344	1	127	17
NEDDylation
RUB1	YDR139C				0	574	24	0	13	4
ULA1	YPL003W				43	8,200	1,513	7	112	31
UBA3	YPR066W				30	9,874	307	5	69	24
UBC12	YLR306W				21	8,458	9,266	2	31	12
DCN1	YLR128W				392	1,301	618	4	23	14
Cullins
RTT101	YJL047C				488	3,358	1,511	14	61	71
CDC53	YDL132W				8	571	242	1	6	7
APC2	YLR127C				417	3,360	1,601	5	20	11
deNEDDylation
LAG2	YOL025W				1143	3,622	1,934	14	59	53
YUH1	YJR099W				520	5,145	838	9	31	22

Among the 20 yeast F-box protein genes, only YLR352W/LUG1 shows a dramatic deficit of insertions in the [URE3] host. The cullin gene RTT101 also showed only rare mutation in the [URE3] host without adenine. DeNEDDylation genes show a modest decrease in the [URE3] host. Frequent insertions in the [URE3] cells grown with adenine may be due to accumulation of [ure-o] cells in these cultures (Table S2, C and D).