| 1 cycle: | 2 min 98°C (initial denaturation) |
| 30 cycles: | 10 sec 95°C (denaturation) |
| 15 sec 60°C (annealing) | |
| 30 sec 72°C (extension) | |
| Final cycle: | 2 min 72°C (final extension) |
| Indefinite 4°C (hold) | |
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