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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Curr Protoc Mol Biol. 2018 Jun 19;123(1):e64. doi: 10.1002/cpmb.64
12.5 μl purified PCR amplicon from step 16 or pSLQ1651-sgRNA(F+E)-sgGal4 vector (1 μg)
1.5 μl 10x NEB buffer 3.1
0.5 μl NEB BstXI
0.5 μl NEB XhoI
water to 15 μl
Incubate reactions at 37°C overnight.