| 5 μl | digested PCR amplicon (100 ng) |
| 1 ul | digested pSLQ1651 backbone (200 ng) |
| 1 μl | 10x NEB T4 ligase buffer |
| 1 μl | NEB T4 ligase |
| water to 10 μl | |
| Incubate at room temperature for 1 h. | |
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