| 1 μg | VSV-G plasmid |
| 2 μg | psPAX2 plasmid |
| 3 μg | pSLQ1651-sgRNA plasmid |
| 18 μg | PEI (Plasmid:PEI ratio is 1:3) |
| 300 μl | Opti-MEM |
| Vortex the tubes to mix, incubate at RT for 20 minus. | |
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