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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2018 May 21;42(7):1166–1176. doi: 10.1111/acer.13762

Figure 3.

Figure 3

Effect of chronic ALC exposure on neurokinin B receptor (NK3R) expression in medial basal hypothalamus (MBH) of prepubertal female rats. (A) Representative Western immunoblot of NK3R and β-actin proteins in the MBH isolated from 29 day-old control (CON; lanes 1-3) and ALC-treated (ALC; lanes 4-6) animals. (B) Densitometric quantitation of all of the bands from two blots assessing NK3R protein levels in the MBH. (C&D) show representative Western immunoblots and densitometric quantitations from 31 day-old rats. These data were normalized to the internal control, β-actin protein, and the densitometric units represent the NK3R/β-actin ratio. Note that NK3R protein levels decreased in the MBH of the ALC-treated animals at 31days of age. The respective bars illustrate the mean (±SEM) of an N of 5-8 in each group. *p<0.05 versus CON.