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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2018 May 21;42(7):1166–1176. doi: 10.1111/acer.13762

Figure 5.

Figure 5

Effect of chronic ALC exposure on the phosphorylation (p) of mammalian target of rapamycin (mTOR) in medial basal hypothalamus (MBH) of prepubertal female rats. (A) Representative Western immunoblot of p-mTOR (Ser 2448) and total mTOR proteins in the MBH isolated from 29 day-old control (CON; lanes 1-3) and ALC-treated (ALC; lanes 4-6) animals. (B) Densitometric quantitation of all of the bands from two blots assessing p-mTOR levels normalized to total mTOR protein expression in the MBH. (C&D) show representative Western immunoblots (CON, lanes 1-3; ALC; lanes 4-6) and densitometric quantitations from 31 day-old rats. Note that p-mTOR protein levels were diminished in the MBH of the ALC-treated animals. The respective bars illustrate the mean (±SEM) of an N of 8-11 in each group. *p<0.05; **p<0.01 versus CON.