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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2018 May 21;42(7):1166–1176. doi: 10.1111/acer.13762

Figure 6.

Figure 6

Effect of chronic ALC exposure on phosphorylation (p) of Akt in medial basal hypothalamus (MBH) of prepubertal female rats. (A) Representative Western immunoblot of p-Akt (Ser473) and total Akt proteins in the MBH isolated from 31 day-old control (CON; lanes 1-3) and ALC-treated (ALC; lanes 4-6) animals. (B) Densitometric quantitation of all of the bands from two blots assessing p-Akt levels normalized to total Akt protein expression in the MBH. Note that p-Akt protein expression decreased in the MBH of the 31 day-old ALC-treated animals. The respective bars illustrate the mean (±SEM) of an N of 8 in each group. ***p<0.001 versus CON.