Figure 12.

Cellular and antibody responses in mice immunized with control liquid formulations and the reconstituted lead lyophilized formulations. (A) CD4+ T cells collected from splenocytes 4 weeks after boost immunization were stimulated with ID93 and assessed for intracellular CD154 and IFN-γ production. (B) Serum antibody isotypes IgG1 and IgG2c were measured from sera collected 4 weeks following boost immunization. Data represent ten mice pooled from two independent experiments with five mice per experiment. Graphs show mean values ± SEM for each group. Statistics by two-way ANOVA with Dunnett’s correction for multiple comparison test relative to the control liquid two vial with no excipient and one-way ANOVA with Sidak’s correction for multiple comparison test within liquid and lyophilized pairs; *P < 0.05; **P < 0.01.

Abbreviations: POC, proof of concept; TP, trehalose–phosphate; TT, trehalose–Tris; TMP, trehalose–mannitol–phosphate; SMP, sucrose–mannitol–phosphate; SP, sucrose–phosphate; ST, sucrose–Tris.