Table 2.
Factors used in DoE construction
| Factor | Type | Range or factors |
|---|---|---|
| DoE1, 40 samples | ||
| Mannitol concentration | Continuous | 0–1% (w/v) |
| Disaccharide concentration | Continuous | 3.5%–10% (w/v) |
| Disaccharide type | Categorical, 2 level | Sucrose, trehalose |
| Buffer type | Categorical, 2 level | Tris, sodium phosphate |
| Solution pH | Categorical, 2 level | 7.5, 8.0 |
| DoE2, 12 samples | ||
| Mannitol concentration | Continuous | 0–1% (w/v) |
| Glycine concentration | Continuous | 0–1% (w/v) |
| DoE3, 15 samples | ||
| Glycine concentration | Continuous | 0–1% (w/v) |
| Disaccharide concentration | Continuous | 2.5%–10% (w/v) |
| Disaccharide type | Categorical, 2 level | Sucrose, trehalose |
Abbreviation: DoE, design of experiments.