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. 2018 Jul 2;9:2558. doi: 10.1038/s41467-018-05007-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — GRSF1 associates with the degradosome. a Mitochondrial proteins identified in all three purifications of hSuv3 or PNPase. Proteins that did not meet the criteria for consideration as putative degradosome-associated are labeled in gray. b Colocalization studies of the degradosome and GRSF1. The degradosome was visualized using a bimolecular fluorescence complementation (BiFC) approach, the principles of which are illustrated schematically. Catalytically active or inactive (hSuv3-G207V, PNPase-R445ER446E) components of the degradosome were transiently expressed in fusion with a split Venus protein. D-foci are not present in every cell due to transfection efficiency below 100%. GRSF1 was detected using immunofluorescence labeling. Specificity of antibodies for GRSF1 was confirmed by their pre-incubation with purified GRSF1 (Supplementary Fig. 1b). Mitochondria were stained with MitoTracker. Scale bar represents 10 µm. c Quantitative analysis of fluorescence microscopy data wherein 35 randomly selected cells were analyzed. Fraction of D-foci co-localizing with GRSF1 was calculated using the object-based colocalization approach. Individual values are shown. Horizontal lines represent mean values. Error bars represent standard deviation. A two-tailed unpaired t-test was applied (***P < 0.0001)