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. 2018 Jul 2;8:9930. doi: 10.1038/s41598-018-27842-y

Figure 2.

Figure 2

Aβ25–35-induced IL-1β production in LPS-primed Bv2 cells is dependent on cathepsin B. (A) Fluorescent images of acridine orange (AO) and a cathepsin B substrate in cells unprimed or primed with LPS (100 ng/ml) alone or primed with LPS plus Aβ25–35 (25 μM) stimulation were exemplified (12 images per condition). Scale bar = 20 μm. (B) LPS-primed cells were treated with different doses (1, 2.5, 5 μM) of cathepsin B inhibitor for 1 h before Aβ25–35 exposure. Amounts of IL-1β in the culture medium after 24 h of Aβ25–35 treatment were measured by ELISA. (C) LPS-primed Bv2 cells were treated with cathepsin B inhibitor (5 μM) for 1 h before Aβ25–35 treatment. Protein expression of caspase-1 was examined by western blotting and quantified by densitometry. Data are shown as mean ± SEM for at least three independent experiments. ***P < 0.001 and ###P < 0.001. AO: acridine orange; Cat B: cathepsin B substrate; Cat B inh: cathepsin B inhibitor.