Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 2;8:9930. doi: 10.1038/s41598-018-27842-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

DAPK1 is involved in Aβ_25–35-induced IL-1β maturation and caspase-1 activation in LPS-primed Bv2 cells. (A) Confirmation of DAPK1 knockdown and cDAPK1 expression in Bv2 cells by western blotting analysis (see original blots in Supplementary Fig. S3A). (B) The effects of DAPK1 knockdown and cDAPK1 expression on Aβ_25–35-induced IL-1β secretion were measured by ELISA. (C–E) The effects of DAPK1 knockdown on LPS-induced TNF-α and IL-6 secretion, as well as LPS + Aβ_25–35-induced LDH release were determined. (F–J) The effects of DAPK1 knockdown and cDAPK1 expression on the expression of caspase-1, pro-IL-1β, NLRP3 and ASC in LPS-primed cells were determined by western blotting analysis (see original blots in Supplementary Fig. S3B). Results are mean ± SEM for at least three independent experiments. ^**P < 0.01, ^***P < 0.001; ^###P < 0.001. cDAPK1: constructively activated DAPK1 construct; Scr: scrambled shRNA; ShDAPK1: DAPK1-specific shRNA.