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. 2018 Jul 2;8:9928. doi: 10.1038/s41598-018-27640-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cleavage products of L1CAM processing vs. detectability. (A) Cleavage products of L1CAM vs. plasmin, KLK8, or plasmin + KLK8 cleavage. Red products are detectable by a C-terminal epitope anti-L1CAM. Bands are numbered sequentially (I–VI) according to descending length from I = full-length. (B) Schematic of hypothetical SDS page of L1CAM cleavage. Cleavage products detectable by C-terminal epitope anti-L1CAM in red. Lane 1: Uncleaved L1CAM; lane 2: L1CAM cleaved by both plasmin and KLK8 (no siRNA interference), including partial cleavage products. Intensity and thicknesses of individual bands would be reduced from that of uncleaved L1CAM, although total intensity and area would hypothetically equal band in lane 1; lane 3: L1CAM cleavage under siRNA blockade of KLK8. Intensity of individual bands would be greater than in lane 2 and sum band density by area would hypothetically equal lane 1; lane 4. (C) “Detectable” (with currently-available antibodies) bands on hypothetical western blot. Lanes are as in B.