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. 2018 Jun 5;28(7):775–778. doi: 10.1038/s41422-018-0050-6

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© IBCB, SIBS, CAS 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Embryonic senescent cells re-enter cell cycle and contribute to tissues after birth. a Whole-mount SAβ-Gal staining on forelimbs of E10.5–P0 mice. Arrowheads indicate SAβ-Gal⁺ cells. b Cartoon image showing SAβ-Gal activity pattern. No SAβ-Gal⁺ cell is detected after E15.5. c Generation of P21-CreER knock-in allele. d Whole-mount immunostaining for P21 or ESR on P21-CreER embryos. e Cartoon image showing expression pattern of P21 and CreER on P21-CreER mouse limbs. f Immunostaining for P21 and ESR on P21-CreER limb sections. g Strategy for genetic lineage tracing by tamoxifen (Tam)-mediated Cre-loxP recombination. h Schematic figure showing experimental strategy. Tam tamoxifen. i Whole-mount and sectional view of tdTomato expression in P21-CreER;R26-tdTomato embryo without tamoxifen (No Tam) treatment. j Immunostaining for tdTomato on E12.5–P0 mouse limb sections. tdTomato⁺ cells persist after birth. k Immunostaining for tdTomato, EdU, and E-cadherin (E-Cad) on E15.5 and E16.5 limb sections. Arrowheads indicate proliferating tdTomato⁺ cells. l Quantification of the percentage of proliferating tdTomato⁺ epithelial cells. n = 5; n.s., nonsignificant. Scale bars: yellow, 1 mm; black, 200 µm; white, 100 µm. Each figure is representative of five individual samples