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. 2018 Jul 2;150(7):949–968. doi: 10.1085/jgp.201711868

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© 2018 Khalili-Araghi et al.

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Figure 17. — D55 is a critical amino acid residue defining transepithelial resistance and ion selectivity for claudin-15 channels. (A) Doxycycline was used to express claudin-15 in MDCK I monolayers stably transfected with tetracycline-inducible EGFP-claudin-15 constructs with and without point mutations. Matched exposures are shown which reveal similar tight junction claudin-15 localization and expression. (B) TER expressed normalized to MDCK I monolayers that were not induced to express claudin-15. (C) Relative permeability of Na⁺ to Cl⁻ (P_Na⁺/P_Cl⁻) shows loss of cation selectivity with mutation of D55. Error bars represent SEM.