Figure 5.

Subcellular localization of deletion mutants of hPop4. VSV-hPop4 constructs were transiently transfected into HEp-2 cells. Cells were fixed with methanol/acetone, and the expressed proteins were visualized by indirect fluorescence microscopy with the use of anti-VSV antibodies (panels 1, 3, 5, and 7). Phase-contrast images of the same cells are shown in panels 2, 4, 6, and 8. Panels 1 and 2: VSV-hPop4; panels 3 and 4: VSV-hPop4 Δ(61–109), nucleolar accumulation; panels 5 and 6: VSV-hPop4 Δ(61–109); accumulation in the Cajal bodies; and panels 7 and 8: VSV-hPop4 Δ(162–220), nucleolar pattern.