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. 2018 Jul 2;150(7):1003–1015. doi: 10.1085/jgp.201812016

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© 2018 Yamada and Strange

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 6. — Functional properties of chimeras containing partial sequence of the LRRC8A IL. (A) Sequences of LRRC8A IL used for constructing 8C-8A(IL) chimeras. Amino acids P147–R262 represents the full-length LRRC8A IL. Region highlighted in green and underlined gave rise to peak swelling-activated currents similar to those observed in cells expressing 8A+8C heteromers. (B) Peak swelling-activated currents observed in various 8C-8A(IL) chimeras. No current was observed in 8C-8A(A207–V231) and 8C-8A(A207–R262) chimeras. GFP-tagged chimeras indicated that these chimeras were likely not expressed in the plasma membrane. Values are means ± SEM (n = 4–7). (C) Time course (top) and current–voltage relationships (bottom left) of resting, swelling-activated, and shrinkage-inactivated currents in Lrrc8^−/− HCT116 cells expressing the 8C-8A(D182–E206) chimera. Values are means ± SEM (n = 7). Bottom right panel shows representative traces of swelling-activated currents induced by the 8C-8A(D182–E206) homomer. (D) Time course (top) and current–voltage relationships (bottom left) of resting, swelling-activated, and shrinkage-inactivated currents in Lrrc8^−/− HCT116 cells expressing the 8E-8A(D182–E206) chimera. Values are means ± SEM (n = 5). Bottom right panel shows representative traces of swelling-activated currents induced by the 8E-8A(D182–E206) homomer.