Table 1. Electrophysiological properties of swelling-activated anion conductances induced by expression of LRRC8 constructs in LRRC8−/− HCT116 cells.
| Construct | Erev | Rectification | Inactivation at +120 mV | τ (ms) at +120 mV | PI/PCl | PGluc/PCl |
|---|---|---|---|---|---|---|
| mV | I+120 mV/I−120 mV | I2s/Imax | ||||
| 8A + 8C | 12.2 ± 0.3 (14) | 2.65 ± 0.14 (7) | 0.70 ± 0.03 (7) | 3,268 ± 684 (7) | 1.5 ± 0.02 (4) | 0.19 ± 0.01 (3) |
| 8A-8C(EL1) | 10.6 ± 0.3 (9) | 2.95 ± 0.21 (5) | 1.01 ± 0.02 (5) | 3,326 ± 1,298 (5) | 2.1 ± 0.06 (4) | 0.17 ± 0.02 (4) |
| P < 0.002 | P < 0.0001 | P < 0.0001 | ||||
| 8C-8A(IL) | 11.5 ± 0.3 (8) | 1.86 ± 0.18 (6) | 0.77 ± 0.06 (6) | 2,507 ± 459 (6) | 2.2 ± 0.04 (4) | 0.26 ± 0.02 (4) |
| P < 0.004 | P < 0.0001 | |||||
| 8A + 8D | 12.6 ± 0.5 (7) | 5.35 ± 0.38 (7) | 0.60 ± 0.04 (7) | 2,206 ± 506 (7) | 1.4 ± 0.03 (5) | 0.26 ± 0.11 (5) |
| 8D-8A(IL) | 9.3 ± 0.2 (10) | 1.98 ± 0.16 (5) | 0.74 ± 0.03 (5) | 1,917 ± 459 (5) | 1.9 ± 0.03 (5) | 0.28 ± 0.02 (4) |
| P < 0.0005 | P < 0.0001 | P < 0.03 | P < 0.0001 | |||
| 8A + 8E | 13.7 ± 0.4 (7) | 2.52 ± 0.13 (6) | 0.23 ± 0.05 (6) | 947 ± 242 (6) | 1.45 ± 0.02 (4) | 0.20 ± 0.01 (4) |
| 8E-8A(IL) | 13.6 ± 0.4 (12) | 5.28 ± 0.20 (6) | 0.09 ± 0.01 (6) | 181 ± 47 (6) | 1.8 ± 0.02 (6) | 0.12 ± 0.003 (5) |
| P < 0.0001 | P < 0.04 | P < 0.01 | P < 0.0001 | P < 0.0001 |
Values are means ± SEM (n). Rectification was estimated as the ratio of peak currents (pA/pF) measured at +120 mV and −120 mV. P values are from comparison of a chimera to the relevant heteromer (e.g., 8A-8C[EL1] and 8C-8A[IL] compared with 8A + 8C).