Table 2. Electrophysiological properties of swelling-activated anion conductances induced by expression of 8C-8A(IL) homomeric chimeras in LRRC8−/− HCT116 cells.
| Construct | Erev | Rectification | Inactivation at +120 mV | τ (ms) at +120 mV |
|---|---|---|---|---|
| mV | I+120 mV/I−120 mV | I2s/Imax | ||
| 8C-8A(IL) | 11.5 ± 0.3 (8) | 1.86 ± 0.18 (6) | 0.77 ± 0.06 (6) | 2,507 ± 459 (6) |
| 8C-8A(P147–E206) | 11.7 ± 0.2 (6) | 1.94 ± 0.15 (6) | 0.61 ± 0.06 (6) | 4,439 ± 870 (5) |
| 8C-8A(P147–S181) | 11.6 ± 0.5 (5) | 1.71 ± 0.13 (5) | 0.35 ± 0.11 (5) | 2,878 ± 1473 (5) |
| P < 0.04 | ||||
| 8C-8A(D182–E206) | 11.5 ± 0.4 (7) | 1.67 ± 0.21 (7) | 0.37 ± 0.05 (7) | 2,748 ± 685 (6) |
| P < 0.003 | ||||
| 8C-8A(D182–N191) | 8.5 ± 0.8 (5) | 1.94 ± 0.22 (5) | 0.46 ± 0.09 (5) | 1,771 ± 372 (4) |
| P < 0.002 | P = 0.07 | |||
| 8C-8A(G192–E206) | 11.2 ± 0.4 (6) | 2.24 ± 0.40 (6) | 0.41 ± 0.06 (6) | 4,118 ± 1,324 (6) |
| P < 0.01 | ||||
| 8C-8A(D182–V231) | 11.0 ± 0.3 (5) | 1.23 ± 0.05 (5) | 0.34 ± 0.04 (5) | 2,224 ± 975 (5) |
| P < 0.0001 | P < 0.002 | |||
| 8E-8A(IL) | 13.6 ± 0.4 (12) | 5.28 ± 0.20 (6) | 0.09 ± 0.01 (6) | 181 ± 47 (6) |
| 8E-8A(D182–E206) | 10.8 ± 0.6 (5) | 5.68 ± 0.26 (5) | 0.10 ± 0.01 (5) | 333 ± 52 (5) |
| P < 0.0001 |
Values are means ± SEM (n). Rectification was estimated as the ratio of peak currents (pA/pF) measured at +120 mV and −120 mV. P values reflect comparisons of the 8C-8A(IL) or 8E-8A(IL) chimeras engineered with the full-length LRRC8A IL with chimeras engineered with partial LRRC8A IL amino sequences.