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. 2018 Jul 2;150(7):1035–1043. doi: 10.1085/jgp.201812015

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© 2018 Tsai and Tsai

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Figure 10. — Patch clamp outside-out recordings of hME. (A and B) Macroscopic recordings with currents >5 pA. (C and D) Recordings with discernible single-channel events. Red curves: Single exponential fit of current recovery after Ru360 removal. Blue bars: 200 nM Ru360. Bath contains 100 mM Ca²⁺. Pipette solutions contain 5 mM EGTA and 5 mM EDTA to reduce free Ca²⁺ below 1 nM to inhibit I_CACC. Voltage was clamped at −80 mV for all recordings.