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. 2018 Jul 2;215(7):1891–1912. doi: 10.1084/jem.20171385

Figure 1.

Figure 1.

Dormant tumor cells may be isolated from spheroid culture using dye staining with subsequent chase. (A) Bright field image of HCT15 spheroids 5 d after seeding. (B) FACS histogram from SW48 spheroid cells after CFSE labeling (blue) and 6 d later (red). (C) Image of HCT15 spheroid 6 d after labeling with CFSE. Single CFSEHigh (LRC) cells indicated. Bars, 100 µm (A and C). (D) FACS histograms of CFSE levels in 6 CRC cell lines 6 d after CFSE labeling. (E) Stacked bar chart showing the cell cycle status of SW948 spheroid cycling and LRCs. (F) Hierarchical clustering of RNAseq data for LRCs from each cell line.