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. 2018 Jul 2;215(7):1891–1912. doi: 10.1084/jem.20171385

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© 2018 Buczacki et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 2. — Dormant CRC cells are a differentiated cell population. (A) GO pathway analysis of genes down-regulated in LRCs. (B) GO pathway analysis of genes up-regulated in LRCs. (C) GSEA charts of significantly enriched gene sets in LRCs and non-LRCs. (D) GSEA showing enrichment of the Krt20 differentiated gene set in LRCs and the Lgr5 CSC gene set in non-LRCs. (E) RT-PCR histogram showing enrichment of Wnt targets in PTK7^High and differentiation markers in PTK7^Low. n = 6; mean ± SEM. ***, P < 0.001; *, P < 0.05 by two-way ANOVA. (F) Bright field images of PTK7^High and PTK7^Low SW948 spheroid cells 5 d after seeding in nonadherent culture. Bars, 100 µm. (G) Histogram of the tumor-initiating cell frequency (TIC) from FACS sorted SW948 and HT55 spheroids. Mean ± SEM. (H) Column scatter plot of xenograft sizes derived from PTK7^High and PTK7^Low SW948 cells. Mean ± SEM; **, P < 0.01 by unpaired t test. (I) FACS histogram of PTK7 levels in LRCs and non-LRCs derived from CFSE-labeled SW948 and HT55 spheroids. (J) Pie charts of the relative proportions of LRCs and non-LRCs within PTK7^High and PTK7^Low populations from SW948 spheroids. Size of each chart is proportional to relative numbers of cells present.