Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 2;215(7):1891–1912. doi: 10.1084/jem.20171385

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Buczacki et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 3. — CCS1-LRCs are differentiated yet can revert to a CSC-like state. (A) Fluorescence micrograph of CFSE-labeled PDO (COLO05) 8 d after labeling with CFSE. LRC marked with arrow. (B) Representative FACS density plot of PTK7 levels in CFSE labeled PDOs. (C) Histogram of B showing the majority of LRCs (CFSE⁺) are differentiated (PTK7^Low). (D) FACS histogram showing the sorting strategy for reseeding of SW948 CFSE-labeled spheroid-derived LRCs and non-LRCs. Accompanying fluorescent microscopy images from seeded wells. Bars, 100 µm (A and D). (E) Replating efficiencies of LRCs and non-LRCs across CCSs. (F) FACS quantification of changes in differentiation status (PTK7 levels) in SW948 spheroid LRCs and cycling cells upon replating in adherent culture.