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. 2018 Jul 2;215(7):1853–1868. doi: 10.1084/jem.20170779

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© 2018 Tanaka et al.

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Figure 3. — Identification of Trim33-target genes during Th17 differentiation. (A) Naive CD4⁺ T cells isolated from WT and Trim33 cKO mice were cultured in Th17 skewing conditions (IL-6, TGF-β, anti–IL-4 antibody, and anti–IFN-γ antibody). The cells were restimulated with anti-CD3 antibody and subjected to microarray analysis. (B) Expression of Th17-related genes in WT and Trim33 KO T cells cultured under Th17 conditions, as cultured in Fig. 2, was assessed by quantitative PCR. Data shown combine results from three independent experiments. Error bars show means ± SD. **, P < 0.01.