Figure 8.

Reciprocal IL-10 regulation by Trim33 and Smad4. (A) Naive CD4⁺ T cells isolated from WT and Trim33 cKO mice were stimulated by anti-CD3 and anti-CD28 antibodies under Th17 skewing conditions. On day 3, the cultured cells and supernatant were harvested, followed by measurement of IL-10 at the mRNA (left) and protein (right) levels. (B) IL-10 production was measured with WT and Smad4 KO Th17 cells, as done in A. (C) Smad4 expression was detected at the mRNA (left) and protein (right) levels in WT and Trim33 KO Th17 cells. β-Actin was used as loading control. Data are representative of three independent experiments. (D) IL-10 production was measured in WT, Trim33 KO, and Trim33 × Smad4 KO Th17 cells, as described in A. (E) IL-17 production by WT, Trim33 KO, and Trim33 × Smad4 KO Th17 cells, after restimulation. Data are representative of two independent experiments. Error bars show means ± SD. **, P < 0.01.