Table 2.
Disease-related proteins assembled using Catcher/Tag technology.
| Category | Antigen | Antigen expression | Platform | Platform expression | Reference |
|---|---|---|---|---|---|
| Self | Peptide-major histocompatibility complex class I multimers | Escherichia coli | Streptavidin-SpyTag, Streptavidin-SpyCatcher | E. coli | (76) |
| Cancer (Neo-self) | EGFRvIII-SpyTag | Chemically synthesized | ΔN1SpyCatcher-AP205 | E. coli | (55) |
| Cancer (Neo-self) | SpyTag-Telomerase | Chemically synthesized | ΔN1SpyCatcher-AP205 | E. coli | (55) |
| Cancer (HPV) | SpyCatcher-E7 | E. coli | Anti-DEC205-SpyTag | Mammalian | (78) |
| Cancer (Breast) | SpyCatcher-HER2 | Insect | SpyTag-AP205-SpyTag | E. coli | (80) |
| Malaria | SpyTag-CIDR | E. coli | ΔN1SpyCatcher-AP205 | E. coli | (55) |
| Malaria | Pfs25-SpyTag | Mammalian | ΔN1SpyCatcher-AP205 | E. coli | (39, 55) |
| Malaria | SpyCatcher-CIDR | Insect | SpyTag-AP205 | E. coli | (75) |
| Malaria | CSP-SpyCatcher | Insect | SpyTag-AP205 | E. coli | (75) |
| Malaria | Pfs25-SpyCatcher | E. coli | SpyTag-AP205-SpyTag | E. coli | (75) |
| Malaria | SpyTag-VAR2CSA | E. coli | SpyCatcher-AP205 | E. coli | (75) |
| Malaria | CSP-SpyCatcher (Full-length 3d7 CSP) | Insect | SpyTag-AP205 | E. coli | (81) |
| Malaria | Simultaneous display of: Pfs25-SpyTag, Pfs28-SnoopTag | Mammalian | ΔN1SpyCatcher-IMX-SnoopCatcher | E. coli | (82) |
| Malaria | SpyCatcher.R0.6C (GLURP fusion of partial Pfs45/48) | Lactococcus lactis | SpyTag-AP205-SpyTag | E. coli | (83) |
| Malaria | SpyCatcher-6C (fragment of Pfs45/48) | L. lactis | SpyTag-AP205-SpyTag | E. coli | (83) |
| Malaria | CSP-SpyCatcher conjugated to SpyTag-DBL1x-DBL2x-ID2a | Insect | N/A | N/A | (84) |
| Pneumococcal | SpyCatcher-(PspA α′-domain)-SnoopTag | E. coli | HbpD(Δd1)-SpyTag | E. coli/Salmonella Typhimurium | (24) |
| Pneumococcal | SpyCatcher-SP1690-SnoopTag | E. coli | HbpD(Δd1)-SpyTag | E. coli/S. Typhimurium | (24) |
| Pneumococcal | SpyCatcher-PsPα-SP1960-SnoopTag | E. coli | HbpD(Δd1)-SpyTag | E. coli/S. Typhimurium | (24) |
| Self | SpyCatcher-IL-5 | Insect | SpyTag-AP205 | E. coli | (75) |
| Self | SpyCatcher-HER2 extracellular domain | Insect | SpyTag-AP205 | E. coli | (75) |
| Self | SpyCatcher-Survivin | Insect | SpyTag-AP205-SpyTag | E. coli | (75) |
| Self | SpyCatcher-IL-5 | Insect | SpyTag-AP205-SpyTag | E. coli | (75) |
| Self | JoTag-transmembrane helices of GPCRs (C5aR)-InCatcher | E. coli | N/A | N/A | (85) |
| Self (heart disease) | PCSK9-SpyCatcher | Insect | SpyTag-AP205 | E. coli | (75) |
| Self (T cell immunity) | CTLA-4-SpyTag | Insect | SpyCatcher-AP205 | E. coli | (75) |
| Self (T cell immunity) | PD-L1-SpyTag | Insect | SpyCatcher-AP205 | E. coli | (75) |
| Tick-borne encephalitis virus | SpyCatcher-OVA8-ED3 | E. coli | Anti-DEC205-SpyTag | Mammalian | (77) |
| Tuberculosis | SpyCatcher-Ag85A | E. coli | SpyTag-AP205 | E. coli | (75) |
N/A, not applicable.