Table 1. Effect of kinesin-1 mutations on survival.
| Khc allele | Description | Lethal stagea | Notes |
|---|---|---|---|
| Control | Knock-in of wild-type kinesin-1 sequence | Not lethal | |
| Null | Q65-stop, protein null | Second instar | Also known as Khc27 (Brendza et al., 1999) |
| Loop 12 swap | Loop 12 swapped for unc-104 loop 12 | Not lethal | Lethal in trans to Khc null allele |
| Loop 11 swap | Loop 11 swapped for unc-104 loop 11 | Not lethal | Not lethal in trans to Khc null allele |
| β5-loop 8 swap | β5-Loop 8 swapped for unc-104 β5-loop 8 | Second instar | Lethal in trans to Khc null allele |
| SKLA | TERF in loop 8 swapped for equivalent unc-104 sequence | Third instar | |
| SQLA | TERF in loop 8 swapped for equivalent Khc-73 sequence | Not lethal | |
| E177K | Charge reversal mutation of E177 in β5-loop 8 | Third instar | E177K knock-in recapitulates mutation in Khc19 (Brendza et al., 1999) |
| E177R | Charge reversal mutation of E177 in β5-loop 8 | Third instar | |
| E177A | Alanine substitution of E177 in β5-loop 8 | Not lethal | Not lethal in trans to Khc null allele |
| S246F | Mutation in loop 11 that disrupts ATP hydrolysis | Pupae | S246F knock-in recapitulates mutation in Khc17 (Brendza et al., 1999) |
| E164K | Mutation in β5-loop 8 that disrupts ATP hydrolysis | Third instar | Also known as Khc23 (Brendza et al., 1999) |
| Δhinge2 | Deletion of hinge 2 in stalk | Third instar | Disrupts autoinhibition, also known as KHCΔH2 (Barlan et al., 2013) |
| R947E | Charge-reversal mutation of R947 in the autoinhibitory IAK motif | Third instar | R947 and E177 form a salt bridge when the motor is autoinhibited (Kaan et al., 2011) |
| P945S | Mutation of P945S in the autoinhibitory IAK motif in the tail | Third instar | Also known as Khc22 (Moua et al., 2011), P945S interacts with F179 when the motor is autoinhibited (Kaan et al., 2011) |
| K944E | Charge-reversal mutation of K944 in the autoinhibitory IAK motif | Third instar | |
| E177K, S246F | Charge reversal mutation of E177 combined with S246 mutation that decreases ATP hydrolysis | Pupae | |
| S181A, S182A | Alanine substitution of serines adjacent to TERF motif | Not lethal | Not lethal in trans to Khc null allele |
| S181D, S182D | Phosphomimetic mutation of serines adjacent to TERF motif | Not lethal | Lethal in trans to Khc null allele |
a
Stage of development when ≥75% of homozygous animals are dead.