Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 2;217(7):2503–2518. doi: 10.1083/jcb.201711037

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Biedka et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Pre-60S subunit protein composition changes dramatically leading up to and during ITS2 processing. (A and B) Sequential pre-60S subunit assembly intermediates were purified from yeast via TAP-tagged AFs Nsa1, Nog2, or Nmd3. The protein composition of the purified pre-60S subunits was analyzed via mass spectrometry (A) and by SDS-PAGE followed by silver staining and Western blotting (B). (C) A working model for the pathway of 60S subunit assembly. The association and dissociation of AFs with pre-60S subunits are indicated as is the timing of pre-rRNA processing events. The lifetimes of the Nsa1, Nog2, and Nmd3 particles are shown; Nsa1 enters preribosomes during early stages of 60S subunit assembly.