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. 2018 Jul 2;217(7):2503–2518. doi: 10.1083/jcb.201711037

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© 2018 Biedka et al.

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Figure 7. — Depletion or mutational inactivation of Las1 results in changes in pre-60S subunit protein composition unlike those observed in other B mutants. (A and B) Preribosomes were purified from yeast expressing (gal) or depleted of (glu) Las1 or from yeast expressing las1R129A or las1H134A using Nop7-TAP as bait. The protein composition of the pre-60S subunits was analyzed by SDS-PAGE followed by silver staining (A) and Western blotting (B). Yeast containing pRS315-las1R129A or pRS315-las1H134A were grown in C-leu minimal media.